Extracellular HIV Tat and Tat cysteine rich peptide increase CCR5 expression in monocytes
نویسندگان
چکیده
منابع مشابه
cloning, expression and library construction for hiv-1 tat protein
background: designing novel therapeutic agents has been a critical challenge for hiv disease. materials and methods: in current study a dna sequence which was encoded the tat protein was synthesized and inserted in pet 28 vector. vector was cloned in bl21-de3 e. coli and cultured in tb media. after protein expression, recombinant tat protein was purified by nta affinity chromatography. the tat ...
متن کاملThrombospondin-1/HIV-1 tat protein interaction: modulation of the biological activity of extracellular Tat.
Tat protein, a trans-activating factor of the human immunodeficiency virus type 1, acts also as an extracellular molecule modulating gene expression, cell survival, growth, transformation, and angiogenesis. Here we demonstrate that human thrombospondin-1 (TSP), a plasma glycoprotein and constituent of the extracellular matrix, binds to glutathione-S-transferase (GST)-Tat protein but not to GST....
متن کاملPentosan polysulfate as an inhibitor of extracellular HIV-1 Tat.
HIV-1 Tat protein, released from HIV-infected cells, may act as a pleiotropic heparin-binding growth factor. From this observation, extracellular Tat has been implicated in the pathogenesis of AIDS and of AIDS-associated pathologies. Here we demonstrate that the heparin analog pentosan polysulfate (PPS) inhibits the interaction of glutathione S-transferase (GST)-Tat protein with heparin immobil...
متن کاملHIV-1 Tat vaccines.
The inexorable spreading of the HIV pandemic and the increasing deaths for AIDS in the developing countries underscore the urgency for an effective, safe and inexpensive vaccine against AIDS. Although many attempts have been made, a candidate vaccine of proven efficacy and safety in non-human primate models is not yet available. This is mostly due to HIV envelope (Env) variability and to the di...
متن کاملTat protein induces human immunodeficiency virus type 1 (HIV-1) coreceptors and promotes infection with both macrophage-tropic and T-lymphotropic HIV-1 strains.
Chemokine receptors CCR5 and CXCR4 are the primary fusion coreceptors utilized for CD4-mediated entry by macrophage (M)- and T-cell line (T)-tropic human immunodeficiency virus type 1 (HIV-1) strains, respectively. Here we demonstrate that HIV-1 Tat protein, a potent viral transactivator shown to be released as a soluble protein by infected cells, differentially induced CXCR4 and CCR5 expressio...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Zhejiang University SCIENCE
سال: 2005
ISSN: 1009-3095
DOI: 10.1631/jzus.2005.b0668